Peptide Reconstitution Guide - Step-by-Step Instructions
Proper reconstitution is essential for maintaining peptide stability and ensuring accurate dosing in research applications. This comprehensive guide covers everything you need to know about reconstituting lyophilized research peptides.
What is Peptide Reconstitution?
Reconstitution is the process of dissolving lyophilized (freeze-dried) peptides in a suitable solvent to create a liquid solution for research use. Most research peptides are supplied in lyophilized form to maximize stability during storage and shipping.
What You'll Need
- Bacteriostatic Water - The most common reconstitution solvent for peptides
- Sterile syringes - For accurate measurement and transfer
- Alcohol swabs - For sterilizing vial tops
- Clean workspace - Minimize contamination risk
- Calculator - For dosing calculations
Step-by-Step Reconstitution Process
Step 1: Prepare Your Workspace
Work in a clean, well-lit area. Wash your hands thoroughly and gather all necessary materials before beginning.
Step 2: Calculate Required Volume
Determine how much bacteriostatic water you need based on your desired concentration. For example, adding 2ml of water to a 10mg peptide vial creates a 5mg/ml solution.
Step 3: Sterilize Vial Tops
Use an alcohol swab to clean the rubber stoppers on both the peptide vial and bacteriostatic water vial.
Step 4: Draw Bacteriostatic Water
Using a sterile syringe, draw the calculated amount of bacteriostatic water from your vial.
Step 5: Add Water to Peptide Vial
Important: Inject the water slowly down the side of the vial, NOT directly onto the lyophilized powder. This prevents peptide degradation from excessive agitation.
Step 6: Gentle Mixing
DO NOT shake the vial. Instead, gently swirl or roll the vial between your palms until the powder is completely dissolved. This may take 1-3 minutes.
Step 7: Inspect the Solution
The reconstituted solution should be clear and free of particles. If you see cloudiness or particles, do not use the solution.
Important Reconstitution Tips
- Never shake - Vigorous shaking can damage peptide bonds
- Use bacteriostatic water - Contains preservatives to prevent bacterial growth
- Inject slowly - Aim for the side of the vial, not the powder
- Allow time to dissolve - Some peptides take longer than others
- Store properly - Refrigerate immediately after reconstitution
Common Reconstitution Volumes
| Peptide Amount | Water Volume | Final Concentration |
|---|---|---|
| 5mg | 2ml | 2.5mg/ml |
| 10mg | 2ml | 5mg/ml |
| 10mg | 1ml | 10mg/ml |
After Reconstitution
Once reconstituted, peptides should be stored in the refrigerator (2-8°C) and used within the timeframe specified in your product documentation. Learn more about proper peptide storage guidelines.
Quality Matters
Always use high-purity peptides for reliable research results. Learn about our purity standards and COA verification.
Ready to Order Research Peptides?
Browse our complete range of high-purity research peptides on our Buy Research Peptides Online (UK) page. All products include bacteriostatic water options.
All products are for research use only. This guide is for informational purposes.